| Description | |
|---|---|
| Catalogue Number | 14-684 |
| Brand Family | Upstate |
| Trade Name |
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| Description | IRAK1 Protein, active, 10 µg |
| Overview | N-terminal 6His-tagged, recombinant, human IRAK1 amino acids 194-end. |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal 6His-tagged, recombinant, human IRAK1 amino acids 194-end. For use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | Human IRAK1 expressed by baculovirus in Sf21 cells. |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Gene Symbol |
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| Protein Target | IRAK1 |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | TKL |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P51617 # Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. COFACTOR: Magnesium. SIZE: 712 amino acids; 76537 Da SUBUNIT: IL-1 stimulation leads to the formation of a signaling complex which dissociates from the IL-1 receptor following the binding of PELI1. Interacts with IL1RL1. TISSUE SPECIFICITY: Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2. PTM: Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation. SIMILARITY: SwissProt: P51617 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 59.7kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of MBP (13-104). |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 6 months at -70°C from date of shipment |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250 µg size --call for pricing and availability and reference catalog number 14-684M when ordering the 250 µg size. |