| Description | |
|---|---|
| Catalogue Number | 14-656 |
| Brand Family | Upstate |
| Trade Name |
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| Description | LIM Kinase 1 Protein, active, 10 µg |
| Overview | N-terminal 6His-tagged, recombinant, human LIM Kinase 1, amino acids 285-638 |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
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| Application | Active, N-terminal 6His-tagged, recombinant, human LIM Kinase 1, amino acids 285-638, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
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| Source | expressed by baculovirus in Sf21 cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. |
| Gene Symbol |
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| Protein Target | LIMK1 |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | TKL |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P53667 # Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development. SIZE: 647 amino acids; 72585 Da SUBUNIT: Self-associates. The LIM domain interacts with the cytoplasmic domain of NRG1. Binds ROCK1. Interacts with SSH1. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle. PTM: Autophosphorylated (By similarity). & Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1. DISEASE: SwissProt: P53667 # Haploinsufficiency of LIMK1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23. SIMILARITY: SwissProt: P53667 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 2 LIM zinc-binding domains. & Contains 1 PDZ (DHR) domain. & Contains 1 protein kinase domain. |
| Molecular Weight | 41.2kDa |
| Product Usage Statements | |
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| Quality Assurance | routinely evaluated by phosphorylation of Cofilin 1 (Catalog# 12-556) |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 6 months at -70°C from date of shipment |
| Packaging Information | |
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| Material Size | 10 µg |