| Description | |
|---|---|
| Catalogue Number | 14-616 |
| Brand Family | Upstate |
| Trade Name |
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| Description | MSK2 Protein, active, 10 µg |
| Overview | N-terminal His6-tagged, recombinant, human MSK2, amino acids 2-end |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal His6-tagged, recombinant, human MSK2, amino acids 2-end, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | expressed by baculovirus in Sf21 insect cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and c-fos. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. |
| Gene Symbol |
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| Protein Target | MSK2 |
| Purification Method | Purified using Ni2+/NTA-agarose. Activated using MAP Kinase 2/Erk2, active (Catalog # 14-173) and repurified using Ni2+/NTA-agarose |
| Target Sub-Family | AGC |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: O75676 # Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. Essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates Ser- 10 of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF) and result in the transcriptional activation of several immediate early genes, including proto- oncogenes FOS and JUN (By similarity). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14). COFACTOR: Magnesium. SIZE: 772 amino acids; 85606 Da SUBUNIT: Forms a complex with either ERK1 or ERK2 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA4 associates with and phosphorylates the NF-kappa-B p65 subunit RELA. SUBCELLULAR LOCATION: Nucleus. SIMILARITY: SwissProt: O75676 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 2 protein kinase domains. MISCELLANEOUS: Enzyme activity requires the presence of both kinase domains (By similarity). |
| Molecular Weight | 89.9kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of 30mM Crosstide (Catalog # 12-331) |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |