| Description | |
|---|---|
| Catalogue Number | 14-596 |
| Brand Family | Upstate |
| Trade Name |
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| Description | LKB1/STRADα/MO25α Protein, active, 10 µg |
| Overview | Recombinant full-length human LKB1 with an N-terminal 6His tag. Provided as a complex with GST-STRADα and GST-MO25α. |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, recombinant full-length human LKB1 with an N-terminal 6His tag. Provided as a complex with GST-STRADα & GST-MO25α. For use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | expressed in Sf21 |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. |
| Gene Symbol |
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| Protein Target | LKB1 |
| Purification Method | GSH agarose followed by Ni2+/NTA agarose |
| Target Sub-Family | CAMK |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q15831 # Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor. COFACTOR: Magnesium or Manganese. SIZE: 433 amino acids; 48636 Da SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2. TISSUE SPECIFICITY: Ubiquitously expressed. Strongest expression in testis and fetal liver. PTM: Phosphorylated by a cAMP-dependent protein kinase (By similarity). DISEASE: SwissProt: Q15831 # Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer. & Defects in STK11 have been associated with testicular tumors [MIM:273300]. It includes germ cell tumor (GCT) or testicular germ cell tumor (TGCT). SIMILARITY: SwissProt: Q15831 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 protein kinase domain. |
| Molecular Weight | 51/76/66kDa |
| Product Usage Statements | |
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| Quality Assurance | Routinely evaluated by phosphorylation of LKBtide. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
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| Material Size | 10 µg |