| Description | |
|---|---|
| Catalogue Number | 14-595 |
| Brand Family | Upstate |
| Trade Name |
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| Description | PP1α Protein, 10 µg |
| Overview | recombinant, full length, human PP1α |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Recombinant, full length, human PP1α, for use in Phosphatase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | expressed in E. coli cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Gene Symbol |
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| Protein Target | PP1α |
| Purification Method | Heparin agarose followed by Q agarose |
| Target Sub-Family | Ser/Thr phosphatase |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P62136 # Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity). SIZE: 330 amino acids; 37512 Da SUBUNIT: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits. PPP1R12A and PPP1R12B mediate binding to myosin. PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen. Interacts with PPP1R9A and PPP1R9B (By similarity). Interacts with PPP1R7. SUBCELLULAR LOCATION: Cytoplasm (By similarity). SIMILARITY: SwissProt: P62136 ## Belongs to the PPP phosphatase family. PP-1 subfamily. |
| Molecular Weight | 37.6kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by dephosphorylation of 100μM DiFMUP |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 6 months at -20°C from date of shipment |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250 µg size --call for pricing and availability and reference catalog number 14-595M when ordering the 250 µg size. |