| Description | |
|---|---|
| Catalogue Number | 14-573 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Pim1 Protein, active, 10 µg |
| Overview | N-terminal GST-tagged recombinant human full length Pim-1 |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal GST-tagged recombinant human full length Pim-1, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | expressed in E. coli cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer (Dhanasekaran et al., 2001 [PubMed 11518967]).[supplied by OMIM] |
| Gene Symbol |
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| Protein Target | Pim-1 |
| Purification Method | Glutathione agarose affinity chromatography |
| Target Sub-Family | CAMK |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P11309 # Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provide a selective advantage in tumorigenesis. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. COFACTOR: Manganese. SIZE: 404 amino acids; 45412 Da SUBUNIT: Binds to RP9. Isoform 2 is isolated as a monomer whereas isoform 1 complexes with other proteins. Isoform 1, but not isoform 2, binds BMX. SUBCELLULAR LOCATION: Isoform 2: Cytoplasm. Nucleus. & Isoform 1: Cell membrane. TISSUE SPECIFICITY: Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines. PTM: Autophosphorylated on both serine/threonine and tyrosine residues. SIMILARITY: SwissProt: P11309 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 62kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of S6 Kinase/Rsk-2 Substrate Peptide 2 |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250 µg size --call for pricing and availability and reference catalog number 14-573M when ordering the 250 µg size. |