| Description | |
|---|---|
| Catalogue Number | 14-558 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | PI3 Kinase (p120γ), 20 µg |
| Overview | N-terminal His6-tagged recombinant full-length human PI 3-kinase (p120γ). |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | For use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | Expressed by baculovirus in Sf21 insect cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a protein that belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. |
| Gene Symbol |
|
| Protein Target | PI3Kγ |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | PI3K/PI4K |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P48736 # 3-phosphorylates the cellular phosphoinositide PtdIns- 4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5- triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production. SIZE: 1102 amino acids; 126454 Da SUBUNIT: Heterodimer of a catalytic subunit (PIK3CG/p120) and a regulatory (PIK3R5a/p101) subunit. TISSUE SPECIFICITY: Pancreas, skeletal muscle, liver and heart. SIMILARITY: SwissProt: P48736 ## Belongs to the PI3/PI4-kinase family. & Contains 1 PI3K/PI4K domain. |
| Molecular Weight | 130kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated in the PIProfiler. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 20 µg |
| Material Package | Also available in 250μg size as catalog # 14-558M - Call for pricing and availability. |