| Description | |
|---|---|
| Catalogue Number | 14-545 |
| Brand Family | Upstate |
| Trade Name |
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| Description | NEK2 Protein, active, 10 µg |
| Overview | N-terminal His6-tagged, recombinant, full-length, human NEK2 |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal His6-tagged, recombinant, full-length, human NEK2, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | expressed by baculovirus in Sf21 cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Gene Symbol |
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| Protein Target | NEK2 |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | Misc |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P51955 # Protein kinase that is involved in mitotic regulation. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not. COFACTOR: Magnesium. SIZE: 445 amino acids; 51763 Da SUBUNIT: Interacts with TERF1. Isoform 1 and isoform 2 form homo- and heterodimers. SUBCELLULAR LOCATION: Nucleus. & Isoform 1: Nucleus, nucleolus. Note=Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end. & Isoform 2: Cytoplasm. Note=Predominantly cytoplasmic. TISSUE SPECIFICITY: Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. DEVELOPMENTAL STAGE: Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Expression of both isoform 1 and isoform 2 is low in the G1 phase and increases in the S/G2 phases. Isoform 1 is absent from cells arrested in the G2/M prometaphase, whereas isoform 2 remains present. SIMILARITY: SwissProt: P51955 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. NIMA subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 52.8kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of MBP |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250 µg size --call for pricing and availability and reference catalog number 14-545M when ordering the 250 µg size. |