| Description | |
|---|---|
| Catalogue Number | 14-542 |
| Brand Family | Upstate |
| Trade Name |
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| Description | MKK7β1 Protein, inactive, 50 µg |
| Overview | N-terminal GST-tagged, recombinant, human MKK7β1 amino acids 2-end |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Inactive, N-terminal GST-tagged, recombinant, human MKK7β1 amino acids 2-end, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | expressed in E. coli |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined. |
| Gene Symbol |
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| Protein Target | MKK7β1 |
| Purification Method | Glutathione-agarose |
| Target Sub-Family | STE |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: O14733 # Stress activated, dual specificity kinase that activates the JUN kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. COFACTOR: Magnesium. SIZE: 419 amino acids; 47485 Da SUBCELLULAR LOCATION: Nucleus. Cytoplasm (By similarity). TISSUE SPECIFICITY: Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle. PTM: Activated by phosphorylation on Ser/Thr. SIMILARITY: SwissProt: O14733 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 74kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of ATF2 (amino acids 19-96) |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 6 months at -20°C |
| Packaging Information | |
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| Material Size | 50 µg |