| Description | |
|---|---|
| Catalogue Number | 14-520 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Casein Kinase 1δ (aa 1-294) Protein, active, 10 µg |
| Overview | N-terminal, GST-tagged, recombinant human CK1 delta, amino acids 1-294 |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal, GST-tagged, recombinant human CK1 delta, amino acids 1-294, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | expressed in E. coli |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene is a member of the casein kinase I (CKI) gene family whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Two transcript variants encoding different isoforms have been found for this gene. |
| Gene Symbol |
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| Protein Target | CK1γδ |
| Purification Method | Glutathione-agarose |
| Target Sub-Family | CK1 |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P48730 # Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. SIZE: 415 amino acids; 47330 Da SUBUNIT: Monomer. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Interacts directly with PER1 and PER2 which may lead to their degradation. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. In blood, highly expressed in hemopioetic cells and mature granulocytes. Also found in monocytes and lymphocytes. PTM: Autophosphorylated on serine and threonine residues (By similarity). DISEASE: "SwissProt: P48730 # Defects in CSNK1D are a cause of familial advanced sleep- phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are morning larks with a 4 hours advance of the sleep, temperature and melatonin rhythms." SIMILARITY: SwissProt: P48730 ## Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 61kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of KRRRALS(p)VASLPGL |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 6 months at -20°C |
| Packaging Information | |
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| Material Size | 10 µg |
| Material Package | Also available in 250 µg size --call for pricing and availability and reference catalog number 14-520M when ordering the 250 µg size. |