| Description | |
|---|---|
| Catalogue Number | 14-511 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Aurora A Protein, active, 10 µg |
| Overview | N-terminal His6-tagged, recombinant full-length human Aurora A |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal His6-tagged, recombinant full-length human Aurora A, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | expressed in Sf21 cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. |
| Gene Symbol |
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| Protein Target | Aurora-A |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | AGC |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: O14965 # May play a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Maybe involved in microtubule formation and/or stabilization. May play a key role during tumor development and progression. SIZE: 403 amino acids; 45809 Da SUBUNIT: Interacts with TACC1 and CPEB1. Interacts with and phosphorylates BORA. SUBCELLULAR LOCATION: Centrosome. Spindle. Note=Localizes on centrosomes in interphase cells and at each spindle pole in mitosis. TISSUE SPECIFICITY: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. Expression is cell-cycle regulated, low in G1/S, accumulates during G2/M, and decreases rapidly after. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.DISEASE:SwissProt: O14965 # Defects in AURKA are responsible for numerical centrosome aberrations including aneuploidy. SIMILARITY: SwissProt: O14965 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 46.9kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of Kemptide |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 6 months at -70C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250μg size as catalog # 14-511M - Call for pricing and availability. |