| Description | |
|---|---|
| Catalogue Number | 14-509 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Rsk1/MAPKAP Kinase 1a Protein, active, Human, 10 µg |
| Overview | N-terminal His6 tagged recombinant full-length human RSK1 |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal His6 tagged recombinant full-length human RSK1, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | expressed in Sf21 cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Gene Symbol |
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| Protein Target | Rsk1 |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | AGC |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q15418 # Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. COFACTOR: Magnesium. SIZE: 735 amino acids; 82723 Da SUBUNIT: Forms a complex with either ERK1 or ERK2 in quiescent cells. Transiently dissociates following mitogenic stimulation. PTM: Autophosphorylated on Ser-380, as part of the activation process. SIMILARITY: SwissProt: Q15418 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 2 protein kinase domains. |
| Molecular Weight | 84kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of MAPKAP Kinase 2 Substrate Peptide |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 6 months at -70C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250μg size as catalog # 14-509M - Call for pricing and availability. |