| Description | |
|---|---|
| Catalogue Number | 14-480 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Rsk2/MAPKAP Kinase 1b Protein, active, 10 µg |
| Overview | Recombinant human RSK2, full-length with an N-terminal His6 tag between Met1 and Pro2 |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, recombinant human RSK2, full-length with an N-terminal His6 tag between Met1 & Pro2, for use in Kinase Assays. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Source | Expressed by baculovirus in Sf21 insect cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). |
| Gene Symbol |
|
| Protein Target | Rsk2 |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | AGC |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P51812 # Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. COFACTOR: Magnesium (By similarity). SIZE: 740 amino acids; 83736 Da SUBUNIT: Forms a complex with either ERK1 or ERK2 in quiescent cells. Transiently dissociates following mitogenic stimulation (By similarity). TISSUE SPECIFICITY: Expressed in many tissues, highest levels in skeletal muscle. PTM: Autophosphorylated on Ser-386, as part of the activation process (By similarity). & Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369 (By similarity). DISEASE: SwissProt: P51812 # Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations. SIMILARITY: SwissProt: P51812 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 2 protein kinase domains. |
| Molecular Weight | 88kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | the MAPKAP Kinase 2 substrate peptide |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 1 year at -70C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250 µg size --call for pricing and availability and reference catalog number 14-480M when ordering the 250 µg size. |