| Description | |
|---|---|
| Catalogue Number | 14-462 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Rsk3 Protein, active, 10 µg |
| Overview | N-terminal His6-tagged recombinant full-length, human RSK3 |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal His6-tagged recombinant full-length, human RSK3, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | expressed in Sf21 insect cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Gene Symbol |
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| Protein Target | Rsk3 |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | AGC |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q15349 # Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. COFACTOR: Magnesium. SIZE: 733 amino acids; 83239 Da SUBUNIT: Forms a complex with either ERK1 or ERK2 in quiescent cells. Transiently dissociates following mitogenic stimulation (By similarity). SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed in many tissues. Highest expression in lung and skeletal muscle. PTM: Autophosphorylated on Ser-377, as part of the activation process (By similarity). SIMILARITY: SwissProt: Q15349 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 2 protein kinase domains. |
| Molecular Weight | 87kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of the MAPKAP Kinase 2 substrate peptide |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 1 year at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250 µg size --call for pricing and availability and reference catalog number 14-462M when ordering the 250 µg size. |