| Description | |
|---|---|
| Catalogue Number | 14-444 |
| Brand Family | Upstate |
| Trade Name |
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| Description | PKC θ Protein, active, 10 µg |
| Overview | Recombinant full-length human PKC theta with a C-terminal 6His-tag PKC Lipid activator (Catalog # 20-133) is required for use. |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, recombinant full-length human PKC theta with a C-terminal 6His-tag. For use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | expressed in Sf21 cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. |
| Gene Symbol |
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| Protein Target | PKCθ |
| Purification Method | immobilized nickel affinity chromatography |
| Target Sub-Family | AGC |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q04759 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. COFACTOR: Magnesium. SIZE: 706 amino acids; 81865 Da SUBUNIT: Interacts with TXNL2/PICOT. TISSUE SPECIFICITY: Skeletal muscle, megakaryoblastic cells and platelets. DOMAIN: SwissProt: Q04759 The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. PTM: Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation. SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain. |
| Molecular Weight | 83kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of Histone H1 substrate in vitro |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 6 months at -70 °C |
| Packaging Information | |
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| Material Size | 10 µg |