| Description | |
|---|---|
| Catalogue Number | 14-439 |
| Brand Family | Upstate |
| Trade Name |
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| Description | MAP Kinase 1/Erk1 Protein, active, 10 µg |
| Overview | Recombinant, full-length, human MAP Kinase 1/Erk1 containing an N-terminal GST tag |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, recombinant, full-length, human MAP Kinase 1/Erk1 containing an N-terminal GST tag, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | Expressed in E. coli |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. |
| Gene Symbol |
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| Protein Target | MAPK1 |
| Purification Method | Purified by glutathione-agarose. Activated with MEK1 and repurified on Ni2+/NTA agarose |
| Target Sub-Family | CMGC |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). COFACTOR: Magnesium (By similarity). SIZE: 360 amino acids; 41390 Da SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4. DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 69.9kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of MBP |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 1 year at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250μg size as catalog # 14-439M - Call for pricing and availability. |