| Description | |
|---|---|
| Catalogue Number | 14-329 |
| Brand Family | Upstate |
| Trade Name |
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| Description | JNK2α2/SAPK1a Protein, active, 10 µg |
| Overview | N-terminal His-tagged, full length human JNK2α2/SAPK1a |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal His-tagged, full length human JNK2α2/SAPK1a, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | Expressed in Sf21 cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this genes mouse counterpart suggest a key role in T-cell differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. |
| Gene Symbol |
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| Protein Target | JNK2α2/SAPK1a |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | CMGC |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P45984 # JNK2 isoforms display different binding patterns: alpha- 1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. COFACTOR: Magnesium. SIZE: 424 amino acids; 48139 Da SUBUNIT: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. DOMAIN: SwissProt: P45984 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. Autophosphorylated in vitro. SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 49.2kDa |
| Product Usage Statements | |
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| Quality Assurance | Routinely evaluated by phosphorylation of ATF2 (amino acids 19-96) |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
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| Material Size | 10 µg |
| Material Package | Also available in 250 µg size --call for pricing and availability and reference catalog number 14-329M when ordering the 250 µg size. |