| Description | |
|---|---|
| Catalogue Number | 14-306 |
| Brand Family | Upstate |
| Trade Name |
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| Description | GSK3β Protein, active, 10 µg |
| Overview | N-terminal His6-tagged recombinant human GSK3β with an H350L mutation |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal His6-tagged recombinant human GSK3β with an H350L mutation, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | Expressed by baculovirus in Sf21 insect cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).[supplied by OMIM] |
| Gene Symbol |
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| Protein Target | GSK3β |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | CMGC |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P49841 # Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. SIZE: 420 amino acids; 46744 Da SUBUNIT: Monomer (By similarity). Interacts with NIN and CABYR. TISSUE SPECIFICITY: Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. PTM: Phosphorylated by AKT1 and ILK1. SIMILARITY: SwissProt: P49841 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 51kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by phosphorylation of Phospho-Glycogen Synthase Peptide-2 |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-306M when ordering the 250μg size. |