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Key Specifications Table
| Species Reactivity | Key Applications |
|---|---|
| H | WB, RIP |
| Description | |
|---|---|
| Catalogue Number | 03-251 |
| Trade Name |
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| Description | RIPAb+™ IGF2BP2 Antibody |
| Overview | RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA-binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation. IGF2BP2 (Insulin-like growth factor 2 mRNA-binding protein 2) is a member of the IGF-II mRNA-binding protein family that post-transcriptionally regulates IGF2 during developmental stages. IGF2BP1, 2, and 3 bind the UTR region of IGF2 transcripts, and are implicated in the transport of RNA targets, to enable protein synthesis at specific locations in the cytoplasm. Research has shown that the IGF2 gene may be involved in ovarian cancer. |
| Alternate Names |
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| Background Information | Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) is a member of the IGF-II mRNA binding protein family that posttranscriptionally regulates IGF2 during developmental stages. IGF2 mRNA-binding protein family members (IGF2BP1, 2 and 3) bind UTR region of IGF2 transcripts and are implicated in the transport of RNA targets to enable protein synthesis at specific locations in the cytoplasm. Studies have indicated variations in the IGF2BP2 gene may affect fetal glucose metabolism and plays a role in type 2 diabetes. In addition to playing a role in glucose metabolism and dieabetes, the insulin-like growth factor (IGF) signaling axis plays an important role in cancer biology. Research has shown IGF2 to be emerging as an important gene for ovarian cancer. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Anti-IGF2BP2 (Mouse Monoclonal). One vial containing 12.5 µg protein G purified monoclonal in buffer containing 0.1 M Tris-glycine (pH 7.4), 150mM NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Store at -20° C. Concentration: 0.176 mg/mL Normal mouse IgG. One vial containing 125 µg purified mouse IgG in 125 µL storage buffer containing 0.1% sodium azide. Store at -20°C. RIP Primer IGF2. One vial containing 75 μL of 5 μM of each primer specific for human IGF2 mRNA. Store at -20°C. FOR: GCG GCT TCT ACT TCA GCA G REV: CAG GTG TCA TAT TGG AAG AAC |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | IGF2BP2 (Insulin-like growth factor 2 mRNA-binding protein 2) is a member of the IGF-II mRNA-binding protein family that post-transcriptionally regulates IGF2 during developmental stages. |
| Key Applications |
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| Application Notes | Western Blot Analysis: Representative lot data. HepG2 cell lysate was probed with Anti-IGF2BP2, clone 5E10-3E1 (1:20,000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates IGF2BP2 (~66 kDa). Note: An uncharacterized band appears at ~50 kDa in some lysates. |
| Biological Information | |
|---|---|
| Immunogen | Histidine-tagged recombinant protein corresponding to human IGF2BP2 |
| Clone | 5E10-3E1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5 UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]. |
| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Binds to the 5-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs. SUBUNIT STRUCTURE: Interacts with HNRPD and IGF2BP1. SUBCELLULAR LOCATION: Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa. TISSUE SPECIFICITY: Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis. DOMAIN: The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA. MISCELLANEOUS: Autoantibodies against IGF2BP2 are detected in sera from some patients with hepatocellular carcinoma. SEQUENCE SIMILARITIES: Belongs to the RRM IMP/VICKZ family. Contains 4 KH domains. Contains 2 RRM (RNA recognition motif) domains. SEQUENCE CAUTION: The sequence AAH21290.1 differs from that shown. Reason: Erroneous initiation. |
| Molecular Weight | ~66 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | RNA Binding Protein Immunoprecipitation: Representative lot data RIP Lysate prepared from 293 cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 1.25 µg of either a normal mouse IgG or 1.25 µg Anti-IGF2BP2 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700). Successful immunoprecipitation of IGF2BP2-associated RNA was verified by qPCR using RIP Primers IGF2, (Figure 1). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 10 assays |
| Material Package | 10 assays per set. Recommended use: 1.25 μg of antibody per RIP (dependent upon biological context). |