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Ubiquitin activating enzyme E1 (His-tag) human recombinant
Overview
Human recombinant Ubiquitin activating enzyme E1 (His-tag)
Background Information
Ubiquitin activating enzyme E1 is responsible for the first step in the formation of the ubiquitin-protein isopeptide bond. It is a critical component for the initiation of in vitro conjugation reactions. E1 activates ubiquitin by first adenylating, with ATP, the C-terminal glycine carboxyl group of ubiquitin and thenlinking this residue to the sulphydryl side chain moiety of a cysteine residue in E1, by forming a high energy thiol ester bond and liberating free AMP. There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin molecules at one time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. The activated ubiquitin is then transferred to the lysine of target proteins via the E2/E3 conjugation cascade.
Product Information
Format
Purified
Presentation
In liquid containing 50 mM Tris-HCl, pH 7.5, containing 1.0 mM DTT. Frozen solution.
Quality Level
MQ100
Applications
Application
Human recombinant Ubiquitin activating enzyme E1 (His-tag).
Key Applications
Enzyme Assays
Biological Information
Host
E. coli
Source
Full length human ubiquitin-activating enzyme E1 containing a C-terminal His-tag
UniProt Number
P22314
Molecular Weight
~119 kDa
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 12 months at -80°C from date of receipt. Avoid multiple freeze/thaw cycles.
