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Human recombinant SIRT5 (His-tag) expressed in E. Coli
Background Information
SIRT5, along with SIRT 3 and 4, is one of three human mitochondrial sirtuins (homologs of yeast Sir2). SIRT5, which is localized to both the mitochondrial matrix and the intermembrane space, is an NAD+-dependent lysine deacetylase (class III HDAC). SIRT5’s only known physiological target is the intermembrane space protein, cytochrome c, suggesting the possibility of regulatory roles in metabolism and/or apoptosis. SIRT5 has also been shown to deacetylate and thereby upregulate the matrix urea cycle enzyme carbamoyl phosphate synthetase 1. This implies an important role for SIRT5 in ammonia detoxification and adaptation to elevated amino acid catabolism during starvation, calorie restriction, or a high protein diet. SIRT5 is most strongly expressed in thymus, lymphoblasts, and heart muscle cells and its chromosomal location (6p23) has been implicated in chromosomal abnormalities associated with malignancies.
Product Information
Format
Purified
Presentation
In liquid containing 25 mM Tris, pH 7.5, 100 mM NaCl, 5 mM DTT, containing 10% glycerol. Frozen solution.
Quality Level
MQ100
Applications
Application
Human recombinant SIRT5 (His-tag) expressed in E. Coli.
Key Applications
Enzyme Assays
Application Notes
HDAC deacetylase assays
Biological Information
Host
E. coli
Purity
Purity >95% by SDS-PAGE
Source
Recombinant residues 37-310 of human SIRT5 isoform 1, with an N-terminal His-tag
Specific Activity
>200 pmol/min/mg at 37ºC, as determined by a fluorometric activity assay.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the SIRT5 into separate tubes before storing at -80°C.
