Cytiva Pre-Packed Column, 5 mL Vol, 16 mm ID, 2.5 cm L, Polypropylene, 4% Highly Cross-Linked Agarose,

Cytiva Pre-Packed Column, 5 mL Vol, 16 mm ID, 2.5 cm L, Polypropylene, 4% Highly Cross-Linked Agarose, GSTrap FF Format, 16 x 25 mm Bed, <20 mLpm Flow Rate. GSTrap FF columns are pre-packed Glutathione Sepharose fast flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins. GST-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap FF.GST-tagged proteins are eluted under mild, non-denaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The resin, Glutathione Sepharose 4 fast flow, is also available as lab packages and is an excellent choice for scale-up. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTA design or FPLC System - GHC-F (Additional S&H or Hazmat Fees May Apply)